Composite

Part:BBa_K1847015:Design

Designed by: Anna Fomitcheva, Lisa Baumgartner   Group: iGEM15_ETH_Zurich   (2015-08-28)


promoter-RBS-INP-annexin V-RFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 2075
    Illegal NheI site found at 2098
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 475
    Illegal AgeI site found at 2684
    Illegal AgeI site found at 2796
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

RBS BBa_B0032 does not provide enough expression to be detectable. We added RFP to the design to make the bacteria easily detectable with microscope or FACS.

Source

INP was cloned from part BBa_K523013. Annexin was synthetically synthesized and then cloned with the INP.

References